Analysis Note

ControlBrain (Striatum/Substantia Nigra)

Application

Research CategoryNeuroscience

Immunohistochemistry: 1:2,000 on rabbit and guinea pig using an ABC detection method.

Western blot

RIA

Inhibitory studies in vivo and in vitro.

Optimal working dilutions must be determined by the end user.

Detect Dopamine β Hydroxylase using this Anti-Dopamine β Hydroxylase Antibody validated for use in IH, FUNC, RIA & WB.

Research Sub CategoryNeurotransmitters & ReceptorsNeuronal & Glial Markers

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Native enzyme from bovine adrenal glands.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB145

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Unpurified

Rabbit Serum. Lyophilized, no preservatives. Reconstitute with 50 µL of sterile distilled water. Centrifuge to remove any insoluble material.

Specificity

Recognizes Dopamine beta hydroxylase. AB1585 reacts with a single band on Western blots of bovine adrenal homogenates and stains only cells known to contain DBH, such as sympathetic neurons, adrenal medullary cells and central adrenergic neurons. Staining is abolished by preincubation with enzyme.

Storage and Stability

Maintain lyophilized material at -20 to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20 to -70°C in undiluted aliquots for up to six months. Avoid repeated freeze/thaw cycles. Glycerol can be added 1:1 for additional stability.

Target description

69 kDa